Journal: Frontiers in Oncology

Article Title: CD47 Enhances Cell Viability and Migration Ability but Inhibits Apoptosis in Endometrial Carcinoma Cells via the PI3K/Akt/mTOR Signaling Pathway

doi: 10.3389/fonc.2020.01525

Figure Lengend Snippet: CD47 up-regulation inhibited apoptosis in HEC-1A and Ishikawa cells. (A,B) Up-regulation of CD47 inhibited cell apoptosis in Ishikawa cell line. (C,D) CD47 overexpression inhibited cell apoptosis in HEC-1A cell line. * p < 0.05.

Article Snippet: The cell cycle of transfected HEC-1A and Ishikawa cells were analyzed using the Cell Cycle and Apoptosis Analysis Kit (Beyotime Biotechnology, Shanghai, China), according to the manufacturer's protocol.

Techniques: Over Expression

Journal: Oncology letters

Article Title: The antibacterial peptide from Bombyx mori cecropinXJ induced growth arrest and apoptosis in human hepatocellular carcinoma cells.

doi: 10.3892/ol.2016.4601

Figure Lengend Snippet: Figure 3. CecropinXJ arrested the cell cycle of Huh‑7 cells at the S phase. Huh‑7 cells were treated with different concentrations of cecropinXJ (0, 5, 10 and 50 µmol/l) for 24 h. (A) Subsequently, cells were stained with DNA staining solution, and analyzed by fluorescence-activated cell sorting. (B) The percentage of cells in the G0/G1, S and G2/M phases of the cell cycle was expressed as the mean ± standard deviation of triplicate samples. *P<0.05 and **P<0.01. Dip, diploid; Anl, aneupl.

Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA), while dimethyl sulfoxide (DMSO) was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was acquired from, 3,3’-dihexyloxacarbocyanine iodide [DiOC6(3)] was obtained from Sigma-Aldrich (St. Louis, MO, USA), cell cycle staining solution was purchased from Multi Sciences (Lianke) Biotech Co., Ltd. (Hangzhou, China) and Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining apoptosis detection kit was acquired from BestBio (Shanghai, China).

Techniques: Staining, Fluorescence, FACS, Standard Deviation